1WDF

crystal structure of MHV spike protein fusion core

1WDF の概要

• エントリーDOI: 10.2210/pdb1wdf/pdb
• 関連するPDBエントリー: 1WDG
• 分子名称: E2 glycoprotein (2 entities in total)
• 機能のキーワード: mhv, coronavirus, heptad repeat, fusion core, viral entry, viral protein
• 由来する生物種: Murine hepatitis virus; 詳細
• 細胞内の位置: Spike protein S2: Virion membrane ; Single-pass type I membrane protein . Spike protein S1: Virion membrane ; Peripheral membrane protein : P11224
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20911.44

構造登録者

Xu, Y.,Liu, Y.,Lou, Z.,Qin, L.,Li, X.,Bai, Z.,Tien, P.,Gao, G.F.,Rao, Z. (登録日: 2004-05-14, 公開日: 2004-06-15, 最終更新日: 2024-03-13)

主引用文献

Xu, Y.,Liu, Y.,Lou, Z.,Qin, L.,Li, X.,Bai, Z.,Pang, H.,Tien, P.,Gao, G.F.,Rao, Z.
Structural Basis for Coronavirus-mediated Membrane Fusion: CRYSTAL STRUCTURE OF MOUSE HEPATITIS VIRUS SPIKE PROTEIN FUSION CORE
J.Biol.Chem., 279:30514-30522, 2004

PubMed Abstract: The surface transmembrane glycoprotein is responsible for mediating virion attachment to cell and subsequent virus-cell membrane fusion. However, the molecular mechanisms for the viral entry of coronaviruses remain poorly understood. The crystal structure of the fusion core of mouse hepatitis virus S protein, which represents the first fusion core structure of any coronavirus, reveals a central hydrophobic coiled coil trimer surrounded by three helices in an oblique, antiparallel manner. This structure shares significant similarity with both the low pH-induced conformation of influenza hemagglutinin and fusion core of HIV gp41, indicating that the structure represents a fusion-active state formed after several conformational changes. Our results also indicate that the mechanisms for the viral fusion of coronaviruses are similar to those of influenza virus and HIV. The coiled coil structure has unique features, which are different from other viral fusion cores. Highly conserved heptad repeat 1 (HR1) and HR2 regions in coronavirus spike proteins indicate a similar three-dimensional structure among these fusion cores and common mechanisms for the viral fusion. We have proposed the binding regions of HR1 and HR2 of other coronaviruses and a structure model of their fusion core based on our mouse hepatitis virus fusion core structure and sequence alignment. Drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation may be applied to the inhibition of a number of emerging infectious diseases, including severe acute respiratory syndrome.

PubMed: 15123674
DOI: 10.1074/jbc.M403760200

実験手法

X-RAY DIFFRACTION (2.5 Å)