1WCV

Structure of the bacterial chromosome segregation protein Soj

1WCV の概要

• エントリーDOI: 10.2210/pdb1wcv/pdb
• 関連するPDBエントリー: 2BEJ 2BEK
• 分子名称: SEGREGATION PROTEIN, CHLORIDE ION, IODIDE ION, ... (4 entities in total)
• 機能のキーワード: soj, atpase, bacterial, chromosome segregation
• 由来する生物種: THERMUS THERMOPHILUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28272.13

構造登録者

Leonard, T.A.,Butler, P.J.G.,Lowe, J. (登録日: 2004-11-25, 公開日: 2005-01-12, 最終更新日: 2024-05-08)

主引用文献

Leonard, T.A.,Butler, P.J.G.,Lowe, J.
Bacterial Chromosome Segregation: Structure and DNA Binding of the Soj Dimer--A Conserved Biological Switch
Embo J., 24:270-, 2005

PubMed Abstract: Soj and Spo0J of the Gram-negative hyperthermophile Thermus thermophilus belong to the conserved ParAB family of bacterial proteins implicated in plasmid and chromosome partitioning. Spo0J binds to DNA near the replication origin and localises at the poles following initiation of replication. Soj oscillates in the nucleoid region in an ATP- and Spo0J-dependent fashion. Here, we show that Soj undergoes ATP-dependent dimerisation in solution and forms nucleoprotein filaments with DNA. Crystal structures of Soj in three nucleotide states demonstrate that the empty and ADP-bound states are monomeric, while a hydrolysis-deficient mutant, D44A, is capable of forming a nucleotide 'sandwich' dimer. Soj ATPase activity is stimulated by Spo0J or the N-terminal 20 amino-acid peptide of Spo0J. Our analysis shows that dimerisation and activation involving a peptide containing a Lys/Arg is conserved for Soj, ParA and MinD and their modulators Spo0J, ParB and MinE, respectively. By homology to the nitrogenase iron protein and the GTPases Ffh/FtsY, we suggest that Soj dimerisation and regulation represent a conserved biological switch.

PubMed: 15635448
DOI: 10.1038/SJ.EMBOJ.7600530

実験手法

X-RAY DIFFRACTION (1.6 Å)