1WCJ

Conserved Hypothetical Protein TM0487 from Thermotoga maritima

Summary for 1WCJ

• Entry DOI: 10.2210/pdb1wcj/pdb
• Related: 1UWD
• Descriptor: HYPOTHETICAL PROTEIN TM0487 (1 entity in total)
• Functional Keywords: structural genomics, unknown function, contains paad domain, similar to paad protein, unknown activity, alpha/beta fold, jcsg, hypothetical protein, psi, protein structure initiative, joint center for structural genomics
• Biological source: THERMOTOGA MARITIMA
• Total number of polymer chains: 1
• Total formula weight: 11517.34

Authors

Almeida, M.S.,Peti, W.,Herrmann, T.,Wuthrich, K.,Joint Center for Structural Genomics (JCSG) (deposition date: 2004-11-17, release date: 2004-12-14, Last modification date: 2024-05-15)

Primary citation

Almeida, M.S.,Herrmann, T.,Peti, W.,Wilson, I.A.,Wuthrich, K.
NMR Structure of the Conserved Hypothetical Protein Tm0487 from Thermotoga Maritima: Implications for 216 Homologous Duf59 Proteins.
Protein Sci., 14:2880-, 2005

PubMed Abstract: The NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima represents an alpha/beta-topology formed by the regular secondary structures alpha1-beta1-beta2-alpha2-beta3-beta4-alpha3- beta5-3(10)-alpha4, with a small anti-parallel beta-sheet of beta-strands 1 and 2, and a mixed parallel/anti-parallel beta-sheet of beta-strands 3-5. Similar folds have previously been observed in other proteins, with amino acid sequence identity as low as 3% and a variety of different functions. There are also 216 sequence homologs of TM0487, which all have the signature sequence of domains of unknown function 59 (DUF59), for which no three-dimensional structures have as yet been reported. The TM0487 structure thus presents a platform for homology modeling of this large group of DUF59 proteins. Conserved among most of the DUF59s are 13 hydrophobic residues, which are clustered in the core of TM0487. A putative active site of TM0487 consisting of residues D20, E22, L23, T51, T52, and C55 is conserved in 98 of the 216 DUF59 sequences. Asp20 is buried within the proposed active site without any compensating positive charge, which suggests that its pK(a) value may be perturbed. Furthermore, the DUF59 family includes ORFs that are part of a conserved chromosomal group of proteins predicted to be involved in Fe-S cluster metabolism.

PubMed: 16199668
DOI: 10.1110/PS.051755805

Experimental method

SOLUTION NMR