1WC7

FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE

1WC7 の概要

• エントリーDOI: 10.2210/pdb1wc7/pdb
• 分子名称: FAB FRAGMENT OF CATALYTIC ANTIBODY 15A9, LIGHT CHAIN, FAB FRAGMENT OF CATALYTIC ANTIBODY 15A9, HEAVY CHAIN, IODIDE ION, ... (5 entities in total)
• 機能のキーワード: antibody, catalytic antibody, transamination, pyridoxal-phosphate, phosphopyridoxyl-l-alanine
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 96669.23

構造登録者

Golinelli-Pimpaneau, B.,Christen, P. (登録日: 2004-11-09, 公開日: 2005-11-30, 最終更新日: 2024-11-13)

主引用文献

Golinelli-Pimpaneau, B.
Structure of a Pseudomerohedrally Twinned Monoclinic Crystal Form of a Pyridoxal Phosphate-Dependent Catalytic Antibody
Acta Crystallogr.,Sect.D, 61:472-, 2005

PubMed Abstract: The purification, crystallization and structure determination at 2.3 A resolution of the complex of the pyridoxal-5'-phosphate (PLP) dependent catalytic antibody 15A9 with a phosphopyridoxyl-L-alanine (PPL-L-alanine) substrate analogue are described. The crystal belongs to space group P2(1), with two molecules in the asymmetric unit related by non-crystallographic symmetry. The unit-cell parameters are a = 63.5, b = 81.7, c = 79.3 A and beta is fortuitously 90 degrees . Refinement of the structure converged at unacceptably high R factors. Although the traditional analysis of intensity distribution did not indicate twinning, pseudomerohedral twinning was revealed by a newer test based on local intensity differences [Padilla & Yeates (2003), Acta Cryst. D59, 1124-1130]. When the potential twinning operator was included in SHELX, the structure could be satisfactorily refined with a twinning fraction of 0.46, indicating a nearly perfect hemihedrally twinned crystal. One of the active sites is occupied by the phosphopyridoxyl-L-alanine ligand, while one iodide ion mimics the cofactor phosphate group in the other. Four other iodide ions are present in the structure: two are involved in specific intermolecular contacts and two dictate the conformation of the CDRH3 loop in each molecule.

PubMed: 15805602
DOI: 10.1107/S0907444905003331

実験手法

X-RAY DIFFRACTION (2.33 Å)