1WAN

DNA DTA TRIPLEX, NMR, 7 STRUCTURES

1WAN の概要

• エントリーDOI: 10.2210/pdb1wan/pdb
• 分子名称: DNA (5'-D(*AP*GP*AP*TP*AP*GP*AP*AP*CP*CP*CP*CP*TP*TP*CP*TP*AP*TP*CP*TP*TP*AP*TP*AP*TP*CP*TP*(D3)P*TP*CP*TP*T)-3') (1 entity in total)
• 機能のキーワード: triplex, intercalator, deoxyribonucleic acid, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9828.44

構造登録者

Wang, E.,Koshlap, K.M.,Gillespie, P.,Dervan, P.B.,Feigon, J. (登録日: 1996-01-14, 公開日: 1996-07-11, 最終更新日: 2024-05-22)

主引用文献

Wang, E.,Koshlap, K.M.,Gillespie, P.,Dervan, P.B.,Feigon, J.
Solution structure of a pyrimidine-purine-pyrimidine triplex containing the sequence-specific intercalating non-natural base D3.
J.Mol.Biol., 257:1052-1069, 1996

PubMed Abstract: We have used NMR spectroscopy to study a pyrimidine-purine-pyrimidine DNA triplex containing a non-natural base, 1-(2-deoxy-beta-D-ribofuranosyl)-4-(3-benzamido)phenylimidazole (D3), in the third strand. The D3 base has been previously shown to specifically recognize T-A and C-G base-pairs via intercalation on the 3' side (with respect to the purine strand) of the target base pair, instead of forming sequence-specific hydrogen bonds. 1H resonance assignments have been made for the D3 base and most of the non-loop portion of the triplex. The solution structure of the triplex was calculated using restrained molecular dynamics and complete relaxation matrix refinement. The duplex portion of the triplex has an over-all helical structure that is more similar to B-DNA than to A-DNA. The three aromatic rings of the D3 base stack on the bases of all three strands and mimic a triplet. The conformation of the D3 base and its sequence specificity are discussed.

PubMed: 8632468
DOI: 10.1006/jmbi.1996.0223

実験手法

SOLUTION NMR