1WAF

DNA POLYMERASE FROM BACTERIOPHAGE RB69

1WAF の概要

• エントリーDOI: 10.2210/pdb1waf/pdb
• 分子名称: DNA POLYMERASE, GUANOSINE (2 entities in total)
• 機能のキーワード: nucleotidyltransferase, rb69 dna polymerase (gp43)
• 由来する生物種: Enterobacteria phage RB69
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 210052.79

構造登録者

Wang, J.,Satter, A.K.M.A.,Wang, C.C.,Karam, J.D.,Konigsberg, W.H.,Steitz, T.A. (登録日: 1997-04-13, 公開日: 1998-01-14, 最終更新日: 2024-02-14)

主引用文献

Wang, J.,Sattar, A.K.,Wang, C.C.,Karam, J.D.,Konigsberg, W.H.,Steitz, T.A.
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69.
Cell(Cambridge,Mass.), 89:1087-1099, 1997

PubMed Abstract: The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.

PubMed: 9215631
DOI: 10.1016/S0092-8674(00)80296-2

実験手法

X-RAY DIFFRACTION (3.2 Å)