1W9X

Bacillus halmapalus alpha amylase

Summary for 1W9X

• Entry DOI: 10.2210/pdb1w9x/pdb
• Descriptor: ALPHA AMYLASE, 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: hydrolase, amylase, acarbose, bacillus, glycoside hydrolase
• Biological source: BACILLUS HALMAPALUS
• Total number of polymer chains: 1
• Total formula weight: 56855.21

Authors

Davies, G.J.,Brzozowski, A.M.,Dauter, Z.,Rasmussen, M.D.,Borchert, T.V.,Wilson, K.S. (deposition date: 2004-10-20, release date: 2005-02-09, Last modification date: 2023-12-13)

Primary citation

Davies, G.J.,Brzozowski, A.M.,Dauter, Z.,Rasmussen, M.D.,Borchert, T.V.,Wilson, K.S.
Structure of a Bacillus Halmapalus Family 13 Alpha-Amylase, Bha, in Complex with an Acarbose-Derived Nonasaccharide at 2.1 A Resolution
Acta Crystallogr.,Sect.D, 61:190-, 2005

PubMed Abstract: The enzymatic digestion of starch by alpha-amylases is one of the key biotechnological reactions of recent times. In the search for industrial biocatalysts, the family GH13 alpha-amylase BHA from Bacillus halmapalus has been cloned and expressed. The three-dimensional structure at 2.1 A resolution has been determined in complex with the (pseudo)tetrasaccharide inhibitor acarbose. Acarbose is found bound as a nonasaccharide transglycosylation product spanning the -6 to +3 subsites. Careful inspection of electron density suggests that the bound ligand could not have been formed through successive transglycosylations of acarbose and must also have featured maltose or maltooligosaccharides as an acceptor.

PubMed: 15681870
DOI: 10.1107/S0907444904027118

Experimental method

X-RAY DIFFRACTION (2.1 Å)