1W9X

Bacillus halmapalus alpha amylase

1W9X の概要

• エントリーDOI: 10.2210/pdb1w9x/pdb
• 分子名称: ALPHA AMYLASE, 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: hydrolase, amylase, acarbose, bacillus, glycoside hydrolase
• 由来する生物種: BACILLUS HALMAPALUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56855.21

構造登録者

Davies, G.J.,Brzozowski, A.M.,Dauter, Z.,Rasmussen, M.D.,Borchert, T.V.,Wilson, K.S. (登録日: 2004-10-20, 公開日: 2005-02-09, 最終更新日: 2023-12-13)

主引用文献

Davies, G.J.,Brzozowski, A.M.,Dauter, Z.,Rasmussen, M.D.,Borchert, T.V.,Wilson, K.S.
Structure of a Bacillus Halmapalus Family 13 Alpha-Amylase, Bha, in Complex with an Acarbose-Derived Nonasaccharide at 2.1 A Resolution
Acta Crystallogr.,Sect.D, 61:190-, 2005

PubMed Abstract: The enzymatic digestion of starch by alpha-amylases is one of the key biotechnological reactions of recent times. In the search for industrial biocatalysts, the family GH13 alpha-amylase BHA from Bacillus halmapalus has been cloned and expressed. The three-dimensional structure at 2.1 A resolution has been determined in complex with the (pseudo)tetrasaccharide inhibitor acarbose. Acarbose is found bound as a nonasaccharide transglycosylation product spanning the -6 to +3 subsites. Careful inspection of electron density suggests that the bound ligand could not have been formed through successive transglycosylations of acarbose and must also have featured maltose or maltooligosaccharides as an acceptor.

PubMed: 15681870
DOI: 10.1107/S0907444904027118

実験手法

X-RAY DIFFRACTION (2.1 Å)