1W9H

The Structure of a Piwi protein from Archaeoglobus fulgidus.

1W9H の概要

• エントリーDOI: 10.2210/pdb1w9h/pdb
• 分子名称: HYPOTHETICAL PROTEIN AF1318, CADMIUM ION, NICKEL (II) ION, ... (5 entities in total)
• 機能のキーワード: argonaute, piwi domain, rnai, risc, nuclear protein
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49962.53

構造登録者

Parker, J.S.,Roe, S.M.,Barford, D. (登録日: 2004-10-13, 公開日: 2005-01-13, 最終更新日: 2024-05-08)

主引用文献

Parker, J.S.,Roe, S.M.,Barford, D.
Crystal Structure of a Piwi Protein Suggests Mechanisms for Sirna Recognition and Slicer Activity
Embo J., 23:4727-, 2004

PubMed Abstract: RNA silencing regulates gene expression through mRNA degradation, translation repression and chromatin remodelling. The fundamental engines of RNA silencing are RISC and RITS complexes, whose common components are 21-25 nt RNA and an Argonaute protein containing a PIWI domain of unknown function. The crystal structure of an archaeal Piwi protein (AfPiwi) is organised into two domains, one resembling the sugar-binding portion of the lac repressor and another with similarity to RNase H. Invariant residues and a coordinated metal ion lie in a pocket that surrounds the conserved C-terminus of the protein, defining a key functional region in the PIWI domain. Furthermore, two Asp residues, conserved in the majority of Argonaute sequences, align spatially with the catalytic Asp residues of RNase H-like catalytic sites, suggesting that in eukaryotic Argonaute proteins the RNase H-like domain may possess nuclease activity. The conserved region around the C-terminus of the PIWI domain, which is required for small interfering RNA (siRNA) binding to AfPiwi, may function as the receptor site for the obligatory 5' phosphate of siRNAs, thereby specifying the cleavage position of the target mRNA.

PubMed: 15565169
DOI: 10.1038/SJ.EMBOJ.7600488

実験手法

X-RAY DIFFRACTION (1.95 Å)