Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

1W9C

Proteolytic fragment of CRM1 spanning six C-terminal HEAT repeats

1W9C の概要
エントリーDOI10.2210/pdb1w9c/pdb
EMDBエントリー1099
分子名称CRM1 PROTEIN (2 entities in total)
機能のキーワードnuclear protein, exportin 1, nuclear export complex
由来する生物種HOMO SAPIENS (HUMAN)
タンパク質・核酸の鎖数2
化学式量合計72543.50
構造登録者
Petosa, C.,Schoehn, G.,Askjaer, P.,Bauer, U.,Moulin, M.,Steuerwald, U.,Soler-Lopez, M.,Baudin, F.,Mattaj, I.W.,Muller, C.W. (登録日: 2004-10-08, 公開日: 2004-12-03, 最終更新日: 2024-05-08)
主引用文献Petosa, C.,Schoehn, G.,Askjaer, P.,Bauer, U.,Moulin, M.,Steuerwald, U.,Soler-Lopez, M.,Baudin, F.,Mattaj, I.W.,Muller, C.W.
Architecture of Crm1-Exportin 1 Suggests How Cooperativity is Achieved During Formation of a Nuclear Export Complex
Mol.Cell, 16:761-, 2004
Cited by
PubMed Abstract: CRM1/Exportin1 mediates the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran. We present a structural model of human CRM1 based on a combination of X-ray crystallography, homology modeling, and electron microscopy. The architecture of CRM1 resembles that of the import receptor transportin1, with 19 HEAT repeats and a large loop implicated in Ran binding. Residues critical for NES recognition are identified adjacent to the cysteine residue targeted by leptomycin B (LMB), a specific CRM1 inhibitor. We present evidence that a conformational change of the Ran binding loop accounts for the cooperativity of Ran- and substrate binding and for the selective enhancement of CRM1-mediated export by the cofactor RanBP3. Our findings indicate that a single architectural and mechanistic framework can explain the divergent effects of RanGTP on substrate binding by many import and export receptors.
PubMed: 15574331
DOI: 10.1016/J.MOLCEL.2004.11.018
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 1w9c
検証レポート(詳細版)ダウンロードをダウンロード

243083

件を2025-10-15に公開中

PDB statisticsPDBj update infoContact PDBjnumon