1W8X

Structural analysis of PRD1

1W8X の概要

• エントリーDOI: 10.2210/pdb1w8x/pdb
• 関連するPDBエントリー: 1CJD 1GW7 1GW8 1HB5 1HB7 1HB9 1HQN 1HX6
• 分子名称: MAJOR CAPSID PROTEIN (PROTEIN P3), PROTEIN P30, PROTEIN P31, ... (4 entities in total)
• 機能のキーワード: virus, p3 major capsid protein, p30 tape measure, p31 penton protein, p16 membrane protein, virus/viral protein, icosahedral virus
• 由来する生物種: ENTEROBACTERIA PHAGE PRD1; 詳細
• 細胞内の位置: Virion: P22535 P27391 P27384; Virion membrane ; Single-pass membrane protein : P27392
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 557106.82

構造登録者

Abrescia, N.G.A.,Cockburn, J.J.B.,Grimes, J.M.,Sutton, G.C.,Diprose, J.M.,Butcher, S.J.,Fuller, S.D.,San Martin, C.,Burnett, R.M.,Stuart, D.I.,Bamford, D.H.,Bamford, J.K.H. (登録日: 2004-10-01, 公開日: 2004-11-11, 最終更新日: 2024-05-08)

主引用文献

Abrescia, N.G.A.,Cockburn, J.J.B.,Grimes, J.M.,Sutton, G.C.,Diprose, J.M.,Butcher, S.J.,Fuller, S.D.,San Martin, C.,Burnett, R.M.,Stuart, D.I.,Bamford, D.H.,Bamford, J.K.H.
Insights Into Assembly from Structural Analysis of Bacteriophage Prd1.
Nature, 432:68-, 2004

PubMed Abstract: The structure of the membrane-containing bacteriophage PRD1 has been determined by X-ray crystallography at about 4 A resolution. Here we describe the structure and location of proteins P3, P16, P30 and P31. Different structural proteins seem to have specialist roles in controlling virus assembly. The linearly extended P30 appears to nucleate the formation of the icosahedral facets (composed of trimers of the major capsid protein, P3) and acts as a molecular tape-measure, defining the size of the virus and cementing the facets together. Pentamers of P31 form the vertex base, interlocking with subunits of P3 and interacting with the membrane protein P16. The architectural similarities with adenovirus and one of the largest known virus particles PBCV-1 support the notion that the mechanism of assembly of PRD1 is scaleable and applies across the major viral lineage formed by these viruses.

PubMed: 15525981
DOI: 10.1038/NATURE03056

実験手法

X-RAY DIFFRACTION (4.2 Å)