1W8A

Third LRR domain of Drosophila Slit

1W8A の概要

• エントリーDOI: 10.2210/pdb1w8a/pdb
• 関連するPDBエントリー: 1OZN
• 分子名称: SLIT PROTEIN (2 entities in total)
• 機能のキーワード: signaling protein, secreted protein, axon guidance, leucine-rich repeat glycoprotein, egf-like domain, signal protein
• 由来する生物種: DROSOPHILA MELANOGASTER (Fruit fly)
• 細胞内の位置: Secreted : P24014
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21313.31

構造登録者

Howitt, J.A.,Clout, N.J.,Hohenester, E. (登録日: 2004-09-17, 公開日: 2004-10-25, 最終更新日: 2024-11-20)

主引用文献

Howitt, J.A.,Clout, N.J.,Hohenester, E.
Binding Site for Robo Receptors Revealed by Dissection of the Leucine-Rich Repeat Region of Slit.
Embo J., 23:4406-, 2004

PubMed Abstract: Recognition of the large secreted protein Slit by receptors of the Robo family provides fundamental signals in axon guidance and other developmental processes. In Drosophila, Slit-Robo signalling regulates midline crossing and the lateral position of longitudinal axon tracts. We report the functional dissection of Drosophila Slit, using structure analysis, site-directed mutagenesis and in vitro assays. The N-terminal region of Slit consists of a tandem array of four independently folded leucine-rich repeat (LRR) domains, connected by disulphide-tethered linkers. All three Drosophila Robos were found to compete for a single highly conserved site on the concave face of the second LRR domain of Slit. We also found that this domain is sufficient for biological activity in a chemotaxis assay. Other Slit activities may require Slit dimerisation mediated by the fourth LRR domain. Our results show that a small portion of Slit is able to induce Robo signalling and indicate that the distinct functions of Drosophila Robos are encoded in their divergent cytosolic domains.

PubMed: 15496984
DOI: 10.1038/SJ.EMBOJ.7600446

実験手法

X-RAY DIFFRACTION (2.8 Å)