1W7J

Crystal Structure Of Myosin V Motor With Essential Light Chain + ADP-BeFx - Near Rigor

1W7J の概要

• エントリーDOI: 10.2210/pdb1w7j/pdb
• 関連するPDBエントリー: 1OE9 1W7I
• 分子名称: MYOSIN VA, MYOSIN LIGHT CHAIN 1, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: motor protein, unconventional myosin, myosin v, chicken, molecular motor, atpase, elc, iq motif, muscle protein, atp-binding, actin- binding, calmodulin-binding, myosin, phosphorylation
• 由来する生物種: GALLUS GALLUS (CHICKEN); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 109214.96

構造登録者

Coureux, P.-D.,Sweeney, H.L.,Houdusse, A. (登録日: 2004-09-03, 公開日: 2005-02-22, 最終更新日: 2023-12-13)

主引用文献

Coureux, P.-D.,Sweeney, H.L.,Houdusse, A.
Three Myosin V Structures Delineate Essential Features of Chemo-Mechanical Transduction
Embo J., 23:4527-, 2004

PubMed Abstract: The molecular motor, myosin, undergoes conformational changes in order to convert chemical energy into force production. Based on kinetic and structural considerations, we assert that three crystal forms of the myosin V motor delineate the conformational changes that myosin motors undergo upon detachment from actin. First, a motor domain structure demonstrates that nucleotide-free myosin V adopts a specific state (rigor-like) that is not influenced by crystal packing. A second structure reveals an actomyosin state that favors rapid release of ADP, and differs from the rigor-like state by a P-loop rearrangement. Comparison of these structures with a third structure, a 2.0 angstroms resolution structure of the motor bound to an ATP analog, illuminates the structural features that provide communication between the actin interface and nucleotide-binding site. Paramount among these is a region we name the transducer, which is composed of the seven-stranded beta-sheet and associated loops and linkers. Reminiscent of the beta-sheet distortion of the F1-ATPase, sequential distortion of this transducer region likely controls sequential release of products from the nucleotide pocket during force generation.

PubMed: 15510214
DOI: 10.1038/SJ.EMBOJ.7600458

実験手法

X-RAY DIFFRACTION (2 Å)