1W6L

3D structure of CotA incubated with CuCl2

Summary for 1W6L

• Entry DOI: 10.2210/pdb1w6l/pdb
• Related: 1GSK 1HKP 1HKZ 1HL0 1HL1 1OF0 1OGR 1UVW 1W6W
• Descriptor: SPORE COAT PROTEIN A, COPPER (II) ION, OXYGEN MOLECULE, ... (5 entities in total)
• Functional Keywords: oxidase, multicopper-oxidase, laccase, oxygen reduction, copper
• Biological source: BACILLUS SUBTILIS
• Total number of polymer chains: 1
• Total formula weight: 59137.25

Authors

Bento, I.,Martins, L.O.,Lopes, G.G.,Carrondo, M.A.,Lindley, P.F. (deposition date: 2004-08-19, release date: 2005-10-26, Last modification date: 2024-10-16)

Primary citation

Bento, I.,Martins, L.O.,Lopes, G.G.,Carrondo, M.A.,Lindley, P.F.
Dioxygen Reduction by Multi-Copper Oxidases; a Structural Perspective.
Dalton Trans., 7:3507-, 2005

PubMed Abstract: The multi-copper oxidases oxidise substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and, following the transfer of four electrons, is reduced to two molecules of water. The precise mechanism of this reduction has been unclear, but recent X-ray structural studies using the CotA endospore coat protein from Bacillus subtilis have given further insights into the principal stages. It is proposed that the mechanism involves binding of the dioxygen into the trinuclear centre so that it is sited approximately symmetrically between the two type 3 copper ions with one oxygen atom close to the type 2 copper ion. Further stages involve the formation of a peroxide intermediate and following the splitting of this intermediate, the migration of the hydroxide moieties towards the solvent exit channel. The migration steps are likely to involve a movement of the type 2 copper ion and its environment. Details of a putative mechanism are described herein based both on structures already reported in the literature and on structures of the CotA protein in the oxidised and reduced states and with the addition of peroxide and the inhibitor, azide.

PubMed: 16234932
DOI: 10.1039/B504806K

Experimental method

X-RAY DIFFRACTION (2 Å)