1W0K

ADP inhibited bovine F1-ATPase

1W0K の概要

• エントリーDOI: 10.2210/pdb1w0k/pdb
• 関連するPDBエントリー: 1BMF 1COW 1E1Q 1E1R 1E79 1EFR 1H8E 1H8H 1NBM 1OHH 1QO1 1W0J
• 分子名称: ATP SYNTHASE ALPHA CHAIN HEART ISOFORM, MITOCHONDRIAL PRECURSOR, ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR, ATP SYNTHASE GAMMA CHAIN, MITOCHONDRIAL PRECURSOR, ... (8 entities in total)
• 機能のキーワード: atp phosphorylase, atp phosphorylase (h+ transporting), atp synthase, f1fo atp synthase, f1-atpase, hydrolase, atp synthesis, atp-binding
• 由来する生物種: BOS TAURUS (BOVINE); 詳細
• 細胞内の位置: Mitochondrion inner membrane (By similarity): P19483; Mitochondrion: P00829 P05631
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 353991.59

構造登録者

Kagawa, R.,Montgomery, M.G.,Braig, K.,Walker, J.E.,Leslie, A.G.W. (登録日: 2004-06-08, 公開日: 2004-07-08, 最終更新日: 2023-12-13)

主引用文献

Kagawa, R.,Montgomery, M.G.,Braig, K.,Leslie, A.G.W.,Walker, J.E.
The Structure of Bovine F1-ATPase Inhibited by Adp and Beryllium Fluoride
Embo J., 23:2734-, 2004

PubMed Abstract: The structure of bovine F1-ATPase inhibited with ADP and beryllium fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP) subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites. However, the ordered water molecule that carries out nucleophilic attack on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP) subunit, strongly indicating that the beta(DP) subunit is the catalytically active conformation. In the structure of F1-ATPase with five bound ADP molecules (three in alpha-subunits, one each in the beta(TP) and beta(DP) subunits), which has also been determined, the conformation of alphaArg373 suggests that it senses the presence (or absence) of the gamma-phosphate of ATP. Two catalytic schemes are discussed concerning the various structures of bovine F1-ATPase.

PubMed: 15229653
DOI: 10.1038/SJ.EMBOJ.7600293

実験手法

X-RAY DIFFRACTION (2.85 Å)