1VZS

Solution structure of subunit F6 from the peripheral stalk region of ATP synthase from bovine heart mitochondria

1VZS の概要

• エントリーDOI: 10.2210/pdb1vzs/pdb
• 分子名称: ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (1 entity in total)
• 機能のキーワード: synthase, atp synthase, peripheral stalk, f6 subunit, hydrogen ion transport
• 由来する生物種: BOS TAURUS (BOVINE)
• 細胞内の位置: Mitochondrion: P02721
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8971.08

構造登録者

Carbajo, R.J.,Silvester, J.A.,Runswick, M.J.,Walker, J.E.,Neuhaus, D. (登録日: 2004-05-25, 公開日: 2004-09-02, 最終更新日: 2024-05-15)

主引用文献

Carbajo, R.J.,Silvester, J.A.,Runswick, M.J.,Walker, J.E.,Neuhaus, D.
Solution Structure of Subunit F(6) from the Peripheral Stalk Region of ATP Synthase from Bovine Heart Mitochondria
J.Mol.Biol., 342:593-, 2004

PubMed Abstract: The ATP synthase enzyme structure includes two stalk assemblies, the central stalk and the peripheral stalk. Catalysis involves rotation of the central stalk assembly together with the membrane-embedded ring of c-subunits driven by the trans-membrane proton-motive force, while the alpha and beta-subunits of F(1) are prevented from co-rotating by their attachment to the peripheral stalk. In the absence of structures of either the intact peripheral stalk or larger complexes containing it, we are studying its individual components and their interactions to build up an overall picture of its structure. Here, we describe an NMR structural characterisation of F(6), which is a 76-residue protein located in the peripheral stalk of the bovine ATP synthase and is essential for coupling between the proton-motive force and catalysis. Isolated F(6) has a highly flexible structure comprising two helices packed together through a loose hydrophobic core and connected by an unstructured linker. Analysis of chemical shifts, (15)N relaxation and RDC measurements confirm that the F(6) structure is flexible on a wide range of timescales ranging from nanoseconds to seconds. The relationship between this structure for isolated F(6) and its role in the intact peripheral stalk is discussed.

PubMed: 15327958
DOI: 10.1016/J.JMB.2004.07.013

実験手法

SOLUTION NMR