1VTG

THE MOLECULAR STRUCTURE OF A DNA-TRIOSTIN A COMPLEX

Summary for 1VTG

• Entry DOI: 10.2210/pdb1vtg/pdb
• Related PRD ID: PRD_000488
• Descriptor: DNA (5'-D(*CP*GP*TP*AP*CP*G)-3'), triostin A, 2-CARBOXYQUINOXALINE (3 entities in total)
• Functional Keywords: bisintercalator, depsipeptide, quinoxaline, antibiotic, antitumor, dna-antibiotic complex, dna/antibiotic
• Biological source: synthetic construct; More
• Total number of polymer chains: 2
• Total formula weight: 2952.51

Authors

Wang, A.H.-J.,Ughetto, G.,Quigley, G.J.,Hakoshima, T.,Van Der Marel, G.A.,Van Boom, J.H.,Rich, A. (deposition date: 1988-08-18, release date: 2011-07-13, Last modification date: 2023-12-27)

Primary citation

Wang, A.H.,Ughetto, G.,Quigley, G.J.,Hakoshima, T.,van der Marel, G.A.,van Boom, J.H.,Rich, A.
The molecular structure of a DNA-triostin A complex.
Science, 225:1115-1121, 1984

PubMed Abstract: The molecular structure of triostin A, a cyclic octadepsipeptide antibiotic, has been solved complexed to a DNA double helical fragment with the sequence CGTACG (C, cytosine; G, guanine; T, thymine; A, adenine). The two planar quinoxaline rings of triostin A bis intercalate on the minor groove of the DNA double helix surrounding the CG base pairs at either end. The alanine residues form hydrogen bonds to the guanines. Base stacking in the DNA is perturbed, and the major binding interaction involves a large number of van der Waals contacts between the peptides and the nucleic acid. The adenine residues in the center are in the syn conformation and are paired to thymine through Hoogsteen base pairing.

PubMed: 6474168

Experimental method

X-RAY DIFFRACTION (1.67 Å)