1VRR

Crystal structure of the restriction endonuclease BstYI complex with DNA

「1YUV」から置き換えられました

1VRR の概要

• エントリーDOI: 10.2210/pdb1vrr/pdb
• 関連するPDBエントリー: 1BHM 1DFM 1SDO
• 分子名称: 5'-D(*TP*TP*AP*TP*AP*GP*AP*TP*CP*TP*AP*TP*AP*A)-3', BstYI (3 entities in total)
• 機能のキーワード: enzyme-dna complex, double helix, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 55001.00

構造登録者

Townson, S.A.,Samuelson, J.C.,Xu, S.Y.,Aggarwal, A.K. (登録日: 2005-06-02, 公開日: 2005-06-07, 最終更新日: 2023-12-27)

主引用文献

Townson, S.A.,Samuelson, J.C.,Xu, S.Y.,Aggarwal, A.K.
Implications for Switching Restriction Enzyme Specificities from the Structure of BstYI Bound to a BglII DNA Sequence.
Structure, 13:791-801, 2005

PubMed Abstract: The type II restriction endonuclease BstYI recognizes the degenerate sequence 5'-RGATCY-3' (where R = A/G and Y = C/T), which overlaps with both BamHI (GGATCC) and BglII (AGATCT), and thus raises the question of whether BstYI DNA recognition will be more BamHI-like or BglII-like. We present here the structure of BstYI bound to a cognate DNA sequence (AGATCT). We find the complex to be more BglII-like with similarities mapping to DNA conformation, domain organization, and residues involved in catalysis. However, BstYI is unique in containing an extended arm subdomain, and the mechanism of DNA capture has both BglII-like and BamHI-like elements. Further, DNA recognition is more minimal than BglII and BamHI, where only two residues mediate recognition of the entire core sequence. Taken together, the structure reveals a mechanism of degenerate DNA recognition and offers insights into the possibilities and limitations in altering specificities of closely related restriction enzymes.

PubMed: 15893669
DOI: 10.1016/j.str.2005.02.018

実験手法

X-RAY DIFFRACTION (2.7 Å)