1VM9

The X-ray Structure of the cys84ala cys85ala double mutant of the [2Fe-2S] Ferredoxin subunit of Toluene-4-Monooxygenase from Pseudomonas Mendocina KR1

1VM9 の概要

• エントリーDOI: 10.2210/pdb1vm9/pdb
• 分子名称: Toluene-4-monooxygenase system protein C, MAGNESIUM ION, FE2/S2 (INORGANIC) CLUSTER, ... (5 entities in total)
• 機能のキーワード: structural genomics, cesg, protein structure initiative, psi, ferredoxin, fes, [2fe-2s] cluster, rieske protein, toluene-4-monooxygenase subunit, center for eukaryotic structural genomics, electron transport
• 由来する生物種: Pseudomonas mendocina
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12552.00

構造登録者

Wesenberg, G.E.,Smith, D.W.,Phillips Jr., G.N.,Bingman, C.A.,Allard, S.T.M.,Moe, L.A.,Fox, B.G.,Center for Eukaryotic Structural Genomics (CESG) (登録日: 2004-09-13, 公開日: 2004-09-21, 最終更新日: 2023-12-27)

主引用文献

Moe, L.A.,Bingman, C.A.,Wesenberg, G.E.,Phillips, G.N.,Fox, B.G.
Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase.
Acta Crystallogr.,Sect.D, 62:476-482, 2006

PubMed Abstract: The structure of the Rieske-type ferredoxin (T4moC) from toluene 4-monooxygenase was determined by X-ray crystallography in the [2Fe-2S](2+) state at a resolution of 1.48 A using single-wavelength anomalous dispersion phasing with the [2Fe-2S] center. The structure consists of ten beta-strands arranged into the three antiparallel beta-sheet topology observed in all Rieske proteins. Trp69 of T4moC is adjacent to the [2Fe-2S] centre, which displaces a loop containing the conserved Pro81 by approximately 8 A away from the [2Fe-2S] cluster compared with the Pro loop in the closest structural and functional homolog, the Rieske-type ferredoxin BphF from biphenyl dioxygenase. In addition, T4moC contains five hydrogen bonds to the [2Fe-2S] cluster compared with three hydrogen bonds in BphF. Moreover, the electrostatic surface of T4moC is distinct from that of BphF. These structural differences are identified as possible contributors to the evolutionary specialization of soluble Rieske-type ferredoxins between the diiron monooxygenases and cis-dihydrodiol-forming dioxygenases.

PubMed: 16627939
DOI: 10.1107/S0907444906006056

実験手法

X-RAY DIFFRACTION (1.48 Å)