1VM1

STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM

「1G56」から置き換えられました

1VM1 の概要

• エントリーDOI: 10.2210/pdb1vm1/pdb
• 関連するPDBエントリー: 1SHV
• 分子名称: BETA-LACTAMASE SHV-1, CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE, TAZOBACTAM INTERMEDIATE, ... (6 entities in total)
• 機能のキーワード: beta-lactamase, beta-lactam hydrolase, penicillinase, detergent binding, inhibitor design, hydrolase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30598.88

構造登録者

Kuzin, A.P.,Nukaga, M.,Nukaga, Y.,Hujer, A.,Bonomo, R.A.,Knox, J.R. (登録日: 2004-08-27, 公開日: 2004-09-07, 最終更新日: 2025-05-07)

主引用文献

Kuzin, A.P.,Nukaga, M.,Nukaga, Y.,Hujer, A.,Bonomo, R.A.,Knox, J.R.
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam.
Biochemistry, 40:1861-1866, 2001

PubMed Abstract: Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.

PubMed: 11327849
DOI: 10.1021/bi0022745

実験手法

X-RAY DIFFRACTION (2.02 Å)