1V9K

The crystal structure of the catalytic domain of pseudouridine synthase RluC from Escherichia coli

1V9K の概要

• エントリーDOI: 10.2210/pdb1v9k/pdb
• 分子名称: Ribosomal large subunit pseudouridine synthase C, SULFATE ION (3 entities in total)
• 機能のキーワード: pseudouridine syntase, rna binding, lyase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52244.34

構造登録者

Machida, Y.,Mizutani, K.,Unzai, S.,Park, S.-Y.,Tame, J.R.H. (登録日: 2004-01-26, 公開日: 2004-05-18, 最終更新日: 2024-10-16)

主引用文献

Mizutani, K.,Machida, Y.,Unzai, S.,Park, S.-Y.,Tame, J.R.H.
Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli
Biochemistry, 43:4454-4463, 2004

PubMed Abstract: The most frequent modification of RNA, the conversion of uridine bases to pseudouridines, is found in all living organisms and often in highly conserved locations in ribosomal and transfer RNA. RluC and RluD are homologous enzymes which each convert three specific uridine bases in Escherichia coli ribosomal 23S RNA to pseudouridine: bases 955, 2504, and 2580 in the case of RluC and 1911, 1915, and 1917 in the case of RluD. Both have an N-terminal S4 RNA binding domain. While the loss of RluC has little phenotypic effect, loss of RluD results in a much reduced growth rate. We have determined the crystal structures of the catalytic domain of RluC, and full-length RluD. The S4 domain of RluD appears to be highly flexible or unfolded and is completely invisible in the electron density map. Despite the conserved topology shared by the two proteins, the surface shape and charge distribution are very different. The models suggest significant differences in substrate binding by different pseudouridine synthases.

PubMed: 15078091
DOI: 10.1021/bi036079c

実験手法

X-RAY DIFFRACTION (2 Å)