1V7F

Solution structure of phrixotoxin 1

1V7F の概要

• エントリーDOI: 10.2210/pdb1v7f/pdb
• 分子名称: Phrixotoxin 1 (1 entity in total)
• 機能のキーワード: toxin
• 由来する生物種: Paraphysa scrofa
• 細胞内の位置: Secreted: P61230
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3559.39

構造登録者

Chagot, B.,Escoubas, P.,Villegas, E.,Bernard, C.,Ferrat, G.,Corzo, G.,Lazdunski, M.,Darbon, H. (登録日: 2003-12-16, 公開日: 2004-11-23, 最終更新日: 2024-10-30)

主引用文献

Chagot, B.,Escoubas, P.,Villegas, E.,Bernard, C.,Ferrat, G.,Corzo, G.,Lazdunski, M.,Darbon, H.
Solution structure of Phrixotoxin 1, a specific peptide inhibitor of Kv4 potassium channels from the venom of the theraphosid spider Phrixotrichus auratus
Protein Sci., 13:1197-1208, 2004

PubMed Abstract: Animal toxins block voltage-dependent potassium channels (Kv) either by occluding the conduction pore (pore blockers) or by modifying the channel gating properties (gating modifiers). Gating modifiers of Kv channels bind to four equivalent extracellular sites near the S3 and S4 segments, close to the voltage sensor. Phrixotoxins are gating modifiers that bind preferentially to the closed state of the channel and fold into the Inhibitory Cystine Knot structural motif. We have solved the solution structure of Phrixotoxin 1, a gating modifier of Kv4 potassium channels. Analysis of the molecular surface and the electrostatic anisotropy of Phrixotoxin 1 and of other toxins acting on voltage-dependent potassium channels allowed us to propose a toxin interacting surface that encompasses both the surface from which the dipole moment emerges and a neighboring hydrophobic surface rich in aromatic residues.

PubMed: 15096626
DOI: 10.1110/ps.03584304

実験手法

SOLUTION NMR