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1V7C

Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue

1UIQ」から置き換えられました
1V7C の概要
エントリーDOI10.2210/pdb1v7c/pdb
関連するPDBエントリー1UIM 1UIN
分子名称THREONINE SYNTHASE, (2E)-2-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]-5-PHOSPHONOPENT-2-ENOIC ACID (3 entities in total)
機能のキーワードplp-dependent enzyme, riken structural genomics/proteomics initiative, rsgi, structural genomics, lyase
由来する生物種Thermus thermophilus
タンパク質・核酸の鎖数4
化学式量合計149923.64
構造登録者
Omi, R.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-12-16, 公開日: 2003-12-30, 最終更新日: 2023-12-27)
主引用文献Omi, R.,Goto, M.,Miyahara, I.,Mizuguchi, H.,Hayashi, H.,Kagamiyama, H.,Hirotsu, K.
Crystal structures of threonine synthase from Thermus thermophilus HB8: conformational change, substrate recognition, and mechanism.
J.Biol.Chem., 278:46035-46045, 2003
Cited by
PubMed Abstract: Threonine synthase, which is a PLP-dependent enzyme, catalyzes the beta,gamma-replacement reaction of l-homoserine phosphate to yield threonine and inorganic phosphate. The three-dimensional structures of the enzyme from Thermus thermophilus HB8 in its unliganded form and complexed with the substrate analogue 2-amino-5-phosphonopentanoic acid have been determined at 2.15 and 2.0 A resolution, respectively. The complexed form, assigned as an enamine, uncovered the interactions of the cofactor-analogue conjugate with the active site residues. The binding of the substrate analogue induces a large conformational change at the domain level. The small domain rotates by about 25 degrees and approaches the large domain to close the active site. The complicated catalytic process of the enzyme has been elucidated based on the complex structure to reveal the stereochemistry of the reaction and to present the released inorganic phosphate as a possible catalyst to carry a proton to the Cgamma atom of the substrate.
PubMed: 12952961
DOI: 10.1074/jbc.M308065200
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2 Å)
構造検証レポート
Validation report summary of 1v7c
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-30に公開中

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