1V75

Crystal structure of hemoglobin D from the Aldabra giant tortoise (Geochelone gigantea) at 2.0 A resolution

Summary for 1V75

• Entry DOI: 10.2210/pdb1v75/pdb
• Descriptor: Hemoglobin D alpha chain, Hemoglobin A and D beta chain, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• Functional Keywords: hemoglobin d, reptilia, the aldabra giant tortoise, geochelone gigantea, oxygen storage-transport complex, oxygen storage/transport
• Biological source: Dipsochelys dussumieri (Aldabra giant tortoise); More
• Total number of polymer chains: 2
• Total formula weight: 33617.98

Authors

Kuwada, T.,Hasegawa, T.,Satoh, I.,Ishikawa, K.,Shishikura, F. (deposition date: 2003-12-12, release date: 2003-12-30, Last modification date: 2023-12-27)

Primary citation

Kuwada, T.,Hasegawa, T.,Satoh, I.,Ishikawa, K.,Shishikura, F.
Crystallization and preliminary X-ray diffraction study of hemoglobin D from the Aldabra giant tortoise, Geochelone gigantea.
Protein Pept.Lett., 10:422-425, 2003

PubMed Abstract: Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 A resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 A, b = 62.4 A, c = 54.0 A, and beta = 110.3 degrees. One alphabeta dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 A(3)Da(-1).

PubMed: 14529497
DOI: 10.2174/0929866033478799

Experimental method

X-RAY DIFFRACTION (2.02 Å)