1V4U

Crystal structure of bluefin tuna carbonmonoxy-hemoglobin

1V4U の概要

• エントリーDOI: 10.2210/pdb1v4u/pdb
• 関連するPDBエントリー: 1V4W 1V4X
• 分子名称: hemoglobin alpha chain, hemoglobin beta chain, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• 機能のキーワード: oxygen transport, heme, respiratory protein, erythrocyte, root effect, swim bladder, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Thunnus thynnus (bluefin tuna); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65943.05

構造登録者

Yokoyama, T.,Chong, K.T.,Miyazaki, Y.,Nakatsukasa, T.,Unzai, S.,Miyazaki, G.,Morimoto, H.,Jeremy, R.H.T.,Park, S.Y. (登録日: 2003-11-19, 公開日: 2004-07-06, 最終更新日: 2024-11-06)

主引用文献

Yokoyama, T.,Chong, K.T.,Miyazaki, G.,Morimoto, H.,Shih, D.T.,Unzai, S.,Tame, J.R.,Park, S.Y.
Novel Mechanisms of pH Sensitivity in Tuna Hemoglobin: A STRUCTURAL EXPLANATION OF THE ROOT EFFECT
J.Biol.Chem., 279:28632-28640, 2004

PubMed Abstract: The crystal structure of hemoglobin has been known for several decades, yet various features of the molecule remain unexplained or controversial. Several animal hemoglobins have properties that cannot be readily explained in terms of their amino acid sequence and known atomic models of hemoglobin. Among these, fish hemoglobins are well known for their widely varying interactions with heterotropic effector molecules and pH sensitivity. Some fish hemoglobins are almost completely insensitive to pH (within physiological limits), whereas others show extremely low oxygen affinity under acid conditions, a phenomenon called the Root effect. X-ray crystal structures of Root effect hemoglobins have not, to date, provided convincing explanations of this effect. Sequence alignments have signally failed to pinpoint the residues involved, and site-directed mutagenesis has not yielded a human hemoglobin variant with this property. We have solved the crystal structure of tuna hemoglobin in the deoxy form at low and moderate pH and in the presence of carbon monoxide at high pH. A comparison of these models shows clear evidence for novel mechanisms of pH-dependent control of ligand affinity.

PubMed: 15117955
DOI: 10.1074/jbc.M401740200

実験手法

X-RAY DIFFRACTION (2 Å)