1V3P

Crystal structure of d(GCGAGAGC): the DNA octaplex structure with I-motif of G-quartet

1V3P の概要

• エントリーDOI: 10.2210/pdb1v3p/pdb
• 関連するPDBエントリー: 1V3N 1V3O
• 分子名称: 5'-D(*GP*(C38)P*GP*AP*GP*AP*GP*C)-3', POTASSIUM ION (3 entities in total)
• 機能のキーワード: octaplex, quadruplex, g-quartet, i-motif, i-motif of g-quartet, base-intercalated duplex, base-intercalated motif, sheared g:a pair, dna, deoxyribonucleic acid, x-ray analysis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5283.28

構造登録者

Kondo, J.,Umeda, S.,Sunami, T.,Takenaka, A. (登録日: 2003-11-03, 公開日: 2004-06-08, 最終更新日: 2023-10-25)

主引用文献

Kondo, J.,Adachi, W.,Umeda, S.,Sunami, T.,Takenaka, A.
Crystal structures of a DNA octaplex with I-motif of G-quartets and its splitting into two quadruplexes suggest a folding mechanism of eight tandem repeats
Nucleic Acids Res., 32:2541-2549, 2004

PubMed Abstract: Recent genomic analyses revealed many kinds of tandem repeats of specific sequences. Some of them are related to genetic diseases, but their biological functions and structures are still unknown. Two X-ray structures of a short DNA fragment d(gcGA[G]1Agc) show that four base-intercalated duplexes are assembled to form an octaplex at a low K+ concentration, in which the eight G5 residues form a stacked double G-quartet in the central part. At a higher K+ concentration, however, the octaplex is split into just two halves. These structural features suggest a folding process of eight tandem repeats of d(ccGA[G]4Agg), according to a double Greek-key motif. Such a packaging of the repeats could facilitate slippage of a certain sequence during DNA replication, to induce increase or decrease of the repeats.

PubMed: 15133122
DOI: 10.1093/nar/gkh575

実験手法

X-RAY DIFFRACTION (2.3 Å)