1V04

serum paraoxonase by directed evolution

1V04 の概要

• エントリーDOI: 10.2210/pdb1v04/pdb
• 分子名称: SERUM PARAOXONASE/ARYLESTERASE 1, CALCIUM ION, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: paraoxonase, hydrolase, directed evolution, antioxidant, israel structural proteomics center, ispc, structural genomics
• 由来する生物種: HOMO SAPIENS; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39779.08

構造登録者

Harel, M.,Aharoni, A.,Gaidukov, L.,Brumshtein, B.,Khersonsky, O.,Yagur, S.,Meged, R.,Dvir, H.,Ravelli, R.B.G.,McCarthy, A.,Toker, L.,Silman, I.,Sussman, J.L.,Tawfik, D.S. (登録日: 2004-03-22, 公開日: 2004-04-23, 最終更新日: 2024-10-23)

主引用文献

Harel, M.,Aharoni, A.,Gaidukov, L.,Brumshtein, B.,Khersonsky, O.,Meged, R.,Dvir, H.,Ravelli, R.B.G.,Mccarthy, A.,Toker, L.,Silman, I.,Sussman, J.L.,Tawfik, D.S.
Structure and Evolution of the Serum Paraoxonase Family of Detoxifying and Anti-Atherosclerotic Enzymes
Nat.Struct.Mol.Biol., 11:412-, 2004

PubMed Abstract: Members of the serum paraoxonase (PON) family have been identified in mammals and other vertebrates, and in invertebrates. PONs exhibit a wide range of physiologically important hydrolytic activities, including drug metabolism and detoxification of nerve agents. PON1 and PON3 reside on high-density lipoprotein (HDL, 'good cholesterol') and are involved in the prevention of atherosclerosis. We describe the first crystal structure of a PON family member, a variant of PON1 obtained by directed evolution, at a resolution of 2.2 A. PON1 is a six-bladed beta-propeller with a unique active site lid that is also involved in HDL binding. The three-dimensional structure and directed evolution studies permit a detailed description of PON1's active site and catalytic mechanism, which are reminiscent of secreted phospholipase A2, and of the routes by which PON family members diverged toward different substrate and reaction selectivities.

PubMed: 15098021
DOI: 10.1038/NSMB767

実験手法

X-RAY DIFFRACTION (2.2 Å)