1UW0
Solution structure of the zinc-finger domain from DNA ligase IIIa
Summary for 1UW0
| Entry DOI | 10.2210/pdb1uw0/pdb |
| Related | 1IMO 1IN1 |
| Descriptor | DNA LIGASE III, ZINC ION (2 entities in total) |
| Functional Keywords | dna repair, zinc finger, ligase, parp-like finger, cell division, dna replication, nuclear protein |
| Biological source | HOMO SAPIENS (HUMAN) |
| Cellular location | Nucleus: P49916 |
| Total number of polymer chains | 1 |
| Total formula weight | 13311.75 |
| Authors | Kulczyk, A.W.,Yang, J.-C.,Neuhaus, D. (deposition date: 2004-01-27, release date: 2004-08-05, Last modification date: 2024-05-15) |
| Primary citation | Kulczyk, A.W.,Yang, J.-C.,Neuhaus, D. Solution Structure and DNA Binding of the Zinc-Finger Domain from DNA Ligase Iiialpha J.Mol.Biol., 341:723-, 2004 Cited by PubMed Abstract: DNA ligase IIIalpha carries out the final ligation step in the base excision repair (BER) and single strand break repair (SSBR) mechanisms of DNA repair. The enzyme recognises single-strand nicks and other damage features in double-stranded DNA, both through the catalytic domain and an N-terminal domain containing a single zinc finger. The latter is homologous to other zinc fingers that recognise damaged DNA, two in the N terminus of poly(adenosine-ribose)polymerase and three in the N terminus of the Arabidopsis thaliana nick-sensing DNA 3'-phosphoesterase. Here, we present the solution structure of the zinc-finger domain of human DNA ligase IIIalpha, the first structure of a finger from this group. It is related to that of the erythroid transcription factor GATA-1, but has an additional N-terminal beta-strand and C-terminal alpha-helix. Chemical shift mapping using a DNA ligand containing a single-stranded break showed that the DNA-binding surface of the DNA-ligase IIIalpha zinc finger is substantially different from that of GATA-1, consistent with the fact that the two proteins recognise very different features in the DNA. Likely implications for DNA binding are discussed. PubMed: 15288782DOI: 10.1016/J.JMB.2004.06.035 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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