1USI

L-leucine-binding protein with phenylalanine bound

1USI の概要

• エントリーDOI: 10.2210/pdb1usi/pdb
• 関連するPDBエントリー: 1USG 1USK 2LBP
• 分子名称: LEUCINE-SPECIFIC BINDING PROTEIN, PHENYLALANINE (3 entities in total)
• 機能のキーワード: leucine-binding protein, protein structure, abc transport systems, transport protein
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Periplasm: P04816
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74489.78

構造登録者

Magnusson, U.,Salopek-Sondi, B.,Luck, L.A.,Mowbray, S.L. (登録日: 2003-11-24, 公開日: 2003-12-18, 最終更新日: 2024-11-13)

主引用文献

Magnusson, U.,Salopek-Sondi, B.,Luck, L.A.,Mowbray, S.L.
X-Ray Structures of the Leucine-Binding Protein Illustrate Conformational Changes and the Basis of Ligand Specificity
J.Biol.Chem., 279:8747-, 2004

PubMed Abstract: The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.

PubMed: 14672931
DOI: 10.1074/JBC.M311890200

実験手法

X-RAY DIFFRACTION (1.8 Å)