1USG

L-leucine-binding protein, apo form

Summary for 1USG

• Entry DOI: 10.2210/pdb1usg/pdb
• Related: 1USI 1USK 2LBP
• Descriptor: LEUCINE-SPECIFIC BINDING PROTEIN (2 entities in total)
• Functional Keywords: leucine-binding protein, protein structure, abc transport systems, transport protein
• Biological source: ESCHERICHIA COLI
• Cellular location: Periplasm: P04816
• Total number of polymer chains: 1
• Total formula weight: 37079.70

Authors

Magnusson, U.,Salopek-Sondi, B.,Luck, L.A.,Mowbray, S.L. (deposition date: 2003-11-24, release date: 2003-12-18, Last modification date: 2024-10-16)

Primary citation

Magnusson, U.,Salopek-Sondi, B.,Luck, L.A.,Mowbray, S.L.
X-Ray Structures of the Leucine-Binding Protein Illustrate Conformational Changes and the Basis of Ligand Specificity
J.Biol.Chem., 279:8747-, 2004

PubMed Abstract: The periplasmic leucine-binding protein is the primary receptor for the leucine transport system in Escherichia coli. We report here the structure of an open ligand-free form solved by molecular replacement and refined at 1.5-A resolution. In addition, two closed ligand-bound structures of the same protein are presented, a phenylalanine-bound form at 1.8 A and a leucine-bound structure at a nominal resolution of 2.4 A. These structures show the basis of this protein's ligand specificity, as well as illustrating the conformational changes that are associated with ligand binding. Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein.

PubMed: 14672931
DOI: 10.1074/JBC.M311890200

Experimental method

X-RAY DIFFRACTION (1.53 Å)