1URH

The "Rhodanese" fold and catalytic mechanism of 3-mercaptopyruvate sulfotransferases: Crystal structure of SseA from Escherichia coli

Summary for 1URH

• Entry DOI: 10.2210/pdb1urh/pdb
• Descriptor: 3-MERCAPTOPYRUVATE SULFURTRANSFERASE, SULFITE ION (3 entities in total)
• Functional Keywords: sulfur-transferase, rhodanese, transferase
• Biological source: ESCHERICHIA COLI
• Cellular location: Cytoplasm (Probable): P31142
• Total number of polymer chains: 2
• Total formula weight: 61563.26

Authors

Spallarossa, A.,Forlani, F.,Carpen, A.,Armirotti, A.,Pagani, S.,Bolognesi, M.,Bordo, D. (deposition date: 2003-10-30, release date: 2003-12-18, Last modification date: 2024-11-06)

Primary citation

Spallarossa, A.,Forlani, F.,Carpen, A.,Armirotti, A.,Pagani, S.,Bolognesi, M.,Bordo, D.
The "Rhodanese" Fold and Catalytic Mechanism of 3-Mercaptopyruvate Sulfurtransferases: Crystal Structure of Ssea from Escherichia Coli
J.Mol.Biol., 335:583-, 2004

PubMed Abstract: 3-Mercaptopyruvate sulfurtransferases (MSTs) catalyze, in vitro, the transfer of a sulfur atom from substrate to cyanide, yielding pyruvate and thiocyanate as products. They display clear structural homology with the protein fold observed in the rhodanese sulfurtransferase family, composed of two structurally related domains. The role of MSTs in vivo, as well as their detailed molecular mechanisms of action have been little investigated. Here, we report the crystal structure of SseA, a MST from Escherichia coli, which is the first MST three-dimensional structure disclosed to date. SseA displays specific structural differences relative to eukaryotic and prokaryotic rhodaneses. In particular, conformational variation of the rhodanese active site loop, hosting the family invariant catalytic Cys residue, may support a new sulfur transfer mechanism involving Cys237 as the nucleophilic species and His66, Arg102 and Asp262 as residues assisting catalysis.

PubMed: 14672665
DOI: 10.1016/J.JMB.2003.10.072

Experimental method

X-RAY DIFFRACTION (2.8 Å)