1UPL

Crystal structure of MO25 alpha

1UPL の概要

• エントリーDOI: 10.2210/pdb1upl/pdb
• 関連するPDBエントリー: 1UPK
• 分子名称: MO25 PROTEIN (2 entities in total)
• 機能のキーワード: transferase, strad, lkb1, armadillo
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80689.97

構造登録者

Milburn, C.C.,Boudeau, J.,Deak, M.,Alessi, D.R.,Van Aalten, D.M.F. (登録日: 2003-10-07, 公開日: 2004-01-22, 最終更新日: 2024-11-13)

主引用文献

Milburn, C.C.,Boudeau, J.,Deak, M.,Alessi, D.R.,Van Aalten, D.M.F.
Crystal Structure of Mo25 Alpha in Complex with the C-Terminus of the Pseudo Kinase Ste-20 Related Adaptor (Strad)
Nat.Struct.Mol.Biol., 11:193-, 2004

PubMed Abstract: Mouse protein 25 alpha (MO25 alpha) is a 40-kDa protein that, together with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase, forms a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase. The latter is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25 alpha binds directly to a conserved Trp-Glu-Phe sequence at the STRAD alpha C terminus, markedly enhancing binding of STRAD alpha to LKB1 and increasing LKB1 catalytic activity. The MO25 alpha crystal structure reveals a helical repeat fold, distantly related to the Armadillo proteins. A complex with the STRAD alpha peptide reveals a hydrophobic pocket that is involved in a unique and specific interaction with the Trp-Glu-Phe motif, further supported by mutagenesis studies. The data represent a first step toward structural analysis of the LKB1-STRAD-MO25 complex, and suggests that MO25 alpha is a scaffold protein to which other regions of STRAD-LKB1, cellular LKB1 substrates or regulatory components could bind.

PubMed: 14730349
DOI: 10.1038/NSMB716

実験手法

X-RAY DIFFRACTION (2.6 Å)