1UP3

Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with METHYL-CELLOBIOSYL-4-DEOXY-4-THIO-BETA-D-CELLOBIOSIDE at 1.6 angstrom

1UP3 の概要

• エントリーDOI: 10.2210/pdb1up3/pdb
• 関連するPDBエントリー: 1UOZ 1UP0 1UP2
• 分子名称: PUTATIVE CELLULASE CEL6, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-methyl beta-D-glucopyranoside, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, family 6
• 由来する生物種: MYCOBACTERIUM TUBERCULOSIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32544.73

構造登録者

Varrot, A.,Leydier, S.,Pell, G.,Gilbert, H.J.,Davies, G.J. (登録日: 2003-09-26, 公開日: 2004-11-18, 最終更新日: 2024-10-16)

主引用文献

Varrot, A.,Leydier, S.,Pell, G.,Macdonald, J.M.,Stick, R.V.,Henrissat, B.,Gilbert, H.J.,Davies, G.J.
Mycobacterium Tuberculosis Strains Possess Functional Cellulases.
J.Biol.Chem., 280:20181-, 2005

PubMed Abstract: The genomes of various Mycobacterium tuberculosis strains encode proteins that do not appear to play a role in the growth or survival of the bacterium in its mammalian host, including some implicated in plant cell wall breakdown. Here we show that M. tuberculosis H37Rv does indeed possess a functional cellulase. The x-ray crystal structure of this enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a highly conserved substrate-binding cleft, which affords similar, and unusual, distortion of the substrate at the catalytic center. The endoglucanase activity, together with the existence of a putative membrane-associated crystalline polysaccharide-binding protein, may reflect the ancestral soil origin of the Mycobacterium or hint at a previously unconsidered environmental niche.

PubMed: 15824123
DOI: 10.1074/JBC.C500142200

実験手法

X-RAY DIFFRACTION (1.6 Å)