1UOJ

CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 IN THE CALCIUM-FREE STATE

Summary for 1UOJ

• Entry DOI: 10.2210/pdb1uoj/pdb
• Related: 1L7L 1OKO
• Descriptor: PA-I GALACTOPHILIC LECTIN, SULFATE ION (3 entities in total)
• Functional Keywords: sugar binding protein, galactose binding
• Biological source: PSEUDOMONAS AERUGINOSA
• Total number of polymer chains: 4
• Total formula weight: 51272.67

Authors

Cioci, G.,Mitchell, E.,Gautier, C.,Wimmerova, M.,Perez, S.,Gilboa-Garber, N.,Imberty, A. (deposition date: 2003-09-19, release date: 2003-12-04, Last modification date: 2023-12-13)

Primary citation

Cioci, G.,Mitchell, E.,Gautier, C.,Wimmerova, M.,Sudakevitz, D.,Perez, S.,Gilboa-Garber, N.,Imberty, A.
Structural Basis of Calcium and Galactose Recognition by the Lectin Pa-Il of Pseudomonas Aeruginosa
FEBS Lett., 555:297-, 2003

PubMed Abstract: The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.

PubMed: 14644431
DOI: 10.1016/S0014-5793(03)01249-3

Experimental method

X-RAY DIFFRACTION (2.4 Å)