1UNK

STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN

1UNK の概要

• エントリーDOI: 10.2210/pdb1unk/pdb
• 分子名称: COLICIN E7 (2 entities in total)
• 機能のキーワード: immunity protein, dimeric structure, rnase active site
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 39627.85

構造登録者

Ko, T.-P.,Hsieh, S.-Y.,Ku, W.-Y.,Tseng, M.-Y.,Chak, K.-F.,Yuan, H.S. (登録日: 1996-06-21, 公開日: 1998-01-07, 最終更新日: 2024-02-14)

主引用文献

Hsieh, S.Y.,Ko, T.P.,Tseng, M.Y.,Ku, W.,Chak, K.F.,Yuan, H.S.
A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7.
EMBO J., 16:1444-1454, 1997

PubMed Abstract: Site-specific cleavage of mRNA has been identified in vivo for the polycistronic colicin E7 operon (ColE7), which occurs between G and A nucleotides located at the Asp52 codon (GAT) of the immunity gene (ceiE7). In vitro, this specific cleavage occurs only in the presence of the ceiE7 gene product (ImmE7). The crystal structure of dimeric ImmE7 has been determined at 1.8 A resolution by X-ray crystallographic analysis. We found that several residues located at the interface of dimeric ImmE7 bear surprising resemblance to the active sites of some RNases. These results suggest that dimeric ImmE7 may possess a novel RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream celE7 gene as well as degradation of the upstream ceaE7 mRNA.

PubMed: 9135159
DOI: 10.1093/emboj/16.6.1444

実験手法

X-RAY DIFFRACTION (1.8 Å)