1UL7

Solution structure of kinase associated domain 1 of mouse MAP/microtubule affinity-regulating kinase 3

1UL7 の概要

• エントリーDOI: 10.2210/pdb1ul7/pdb
• NMR情報: BMRB: 10025
• 分子名称: MAP/microtubule affinity-regulating kinase 3 (1 entity in total)
• 機能のキーワード: ka1 domain, elkl motif, mark3, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, transferase
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11533.26

構造登録者

Tochio, N.,Koshiba, S.,Kigawa, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-09-10, 公開日: 2004-03-10, 最終更新日: 2023-12-27)

主引用文献

Tochio, N.,Koshiba, S.,Kobayashi, N.,Inoue, M.,Yabuki, T.,Aoki, M.,Seki, E.,Matsuda, T.,Tomo, Y.,Motoda, Y.,Kobayashi, A.,Tanaka, A.,Hayashizaki, Y.,Terada, T.,Shirouzu, M.,Kigawa, T.,Yokoyama, S.
Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3
Protein Sci., 15:2534-2543, 2006

PubMed Abstract: Microtubule-associated protein/microtubule affinity-regulating kinases (MARKs)/PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1), although its function is unknown. In this study, we determined the solution structure of the KA1 domain of mouse MARK3 by NMR spectroscopy. We found that approximately 50 additional residues preceding the previously defined KA1 domain are required for its proper folding. The newly defined KA1 domain adopts a compact alpha+beta structure with a betaalphabetabetabetabetaalpha topology. We also found a characteristic hydrophobic, concave surface surrounded by positively charged residues. This concave surface includes the highly conserved Glu-Leu-Lys-Leu motif at the C terminus, indicating that it is important for the function of the KA1 domain.

PubMed: 17075132
DOI: 10.1110/ps.062391106

実験手法

SOLUTION NMR