1UL5

Solution structure of the DNA-binding domain of squamosa promoter binding protein-like 7

1UL5 の概要

• エントリーDOI: 10.2210/pdb1ul5/pdb
• 分子名称: squamosa promoter binding protein-like 7, ZINC ION (2 entities in total)
• 機能のキーワード: transcription factor, sbp, flower development, dna binding protein, structural genomics, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Nucleus (Probable): Q8S9G8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10284.56

構造登録者

Yamasaki, K.,Inoue, M.,Kigawa, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-09-09, 公開日: 2004-03-09, 最終更新日: 2023-12-27)

主引用文献

Yamasaki, K.,Kigawa, T.,Inoue, M.,Tateno, M.,Yamasaki, T.,Yabuki, T.,Aoki, M.,Seki, E.,Matsuda, T.,Nunokawa, E.,Ishizuka, Y.,Terada, T.,Shirouzu, M.,Osanai, T.,Tanaka, A.,Seki, M.,Shinozaki, K.,Yokoyama, S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors.
J.Mol.Biol., 337:49-63, 2004

PubMed Abstract: SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

PubMed: 15001351
DOI: 10.1016/j.jmb.2004.01.015
主引用文献が同じPDBエントリー

実験手法

SOLUTION NMR