1UEK

Crystal structure of 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase

1UEK の概要

• エントリーDOI: 10.2210/pdb1uek/pdb
• 分子名称: 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase (2 entities in total)
• 機能のキーワード: non-mevalonate pathway, ghmp superfamily, riken structural genomics/proteomics initiative, rsgi, structural genomics, transferase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29286.54

構造登録者

Wada, T.,Kuramitsu, S.,Yokoyama, S.,Tame, J.R.H.,Park, S.Y.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-05-17, 公開日: 2003-06-17, 最終更新日: 2023-12-27)

主引用文献

Wada, T.,Kuzuyama, T.,Satoh, S.,Kuramitsu, S.,Yokoyama, S.,Unzai, S.,Tame, J.R.,Park, S.Y.
Crystal Structure of 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an Enzyme in the Non-mevalonate Pathway of Isoprenoid Synthesis.
J.Biol.Chem., 278:30022-30027, 2003

PubMed Abstract: The crystal structure of the enzyme 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME) kinase from the thermophilic bacterium Thermus thermophilus HB8 has been determined at 1.7-A resolution. This enzyme catalyzes phosphorylation of the 2-hydroxyl group of CDP-ME, the fourth step of the non-mevalonate pathway, which is essential for isoprenoid biosynthesis in several pathogenic microorganisms. Since this pathway is absent in humans, it is an important target for the development of novel antimicrobial compounds. The structure of the enzyme is similar to the structures of mevalonate kinase and homoserine kinase, members of the GHMP superfamily. Lys8 and Asp125 are active site residues in mevalonate kinase that also appear to play a catalytic role in CDP-ME kinase. Both the mevalonate and the non-mevalonate pathways therefore involve closely related kinases with similar mechanisms. Assaying the enzyme showed that CDP-ME kinase will phosphorylate CDP-ME but not 4-(uridine 5'-diphospho)-2-C-methyl-D-erythritol, indicating the substrate pyrimidine moiety is involved in important interactions with the enzyme.

PubMed: 12771135
DOI: 10.1074/jbc.M304339200

実験手法

X-RAY DIFFRACTION (1.7 Å)