1UDM

Solution structure of Coactosin-like protein (Cofilin family) from Mus Musculus

1UDM の概要

• エントリーDOI: 10.2210/pdb1udm/pdb
• 分子名称: Coactosin-like protein (1 entity in total)
• 機能のキーワード: actin binding protein, cytoskeletal, riken structural genomics/proteomics initiative, rsgi, structural genomics, protein binding
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): Q9CQI6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16538.46

構造登録者

Goroncy, A.K.,Kigawa, T.,Koshiba, S.,Kobayashi, N.,Tochio, N.,Inoue, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2003-05-01, 公開日: 2004-05-18, 最終更新日: 2023-12-27)

主引用文献

Goroncy, A.K.,Koshiba, S.,Tochio, N.,Tomizawa, T.,Sato, M.,Inoue, M.,Watanabe, S.,Hayashizaki, Y.,Tanaka, A.,Kigawa, T.,Yokoyama, S.
NMR solution structures of actin depolymerizing factor homology domains.
Protein Sci., 18:2384-2392, 2009

PubMed Abstract: Actin is one of the most conserved proteins in nature. Its assembly and disassembly are regulated by many proteins, including the family of actin-depolymerizing factor homology (ADF-H) domains. ADF-H domains can be divided into five classes: ADF/cofilin, glia maturation factor (GMF), coactosin, twinfilin, and Abp1/drebrin. The best-characterized class is ADF/cofilin. The other four classes have drawn much less attention and very few structures have been reported. This study presents the solution NMR structure of the ADF-H domain of human HIP-55-drebrin-like protein, the first published structure of a drebrin-like domain (mammalian), and the first published structure of GMF beta (mouse). We also determined the structures of mouse GMF gamma, the mouse coactosin-like domain and the C-terminal ADF-H domain of mouse twinfilin 1. Although the overall fold of the five domains is similar, some significant differences provide valuable insights into filamentous actin (F-actin) and globular actin (G-actin) binding, including the identification of binding residues on the long central helix. This long helix is stabilized by three or four residues. Notably, the F-actin binding sites of mouse GMF beta and GMF gamma contain two additional beta-strands not seen in other ADF-H structures. The G-actin binding site of the ADF-H domain of human HIP-55-drebrin-like protein is absent and distorted in mouse GMF beta and GMF gamma.

PubMed: 19768801
DOI: 10.1002/pro.248

実験手法

SOLUTION NMR