1UDK

Solution Structure of Nawaprin

1UDK の概要

• エントリーDOI: 10.2210/pdb1udk/pdb
• 分子名称: Nawaprin (1 entity in total)
• 機能のキーワード: antiparallel beta-sheet, spiral backbone configuration, unknown function
• 由来する生物種: Naja nigricollis (spitting cobra)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5300.23

構造登録者

Torres, A.M.,Wong, H.Y.,Desai, M.,Moochhala, S.,Kuchel, P.W.,Kini, R.M. (登録日: 2003-05-01, 公開日: 2003-11-04, 最終更新日: 2024-10-30)

主引用文献

Torres, A.M.,Wong, H.Y.,Desai, M.,Moochhala, S.,Kuchel, P.W.,Kini, R.M.
Identification of a Novel Family of Proteins in Snake Venoms: Purification and Structural Characterization of Nawaprin from Naja nigricollis Snake Venom
J.BIOL.CHEM., 278:40097-40104, 2003

PubMed Abstract: The three-dimensional structure of nawaprin has been determined by nuclear magnetic resonance spectroscopy. This 51-amino acid residue peptide was isolated from the venom of the spitting cobra, Naja nigricollis, and is the first member of a new family of snake venom proteins referred to as waprins. Nawaprin is relatively flat and disc-like in shape, characterized by a spiral backbone configuration that forms outer and inner circular segments. The two circular segments are held together by four disulfide bonds, three of which are clustered at the base of the molecule. The inner segment contains a short antiparallel beta-sheet, whereas the outer segment is devoid of secondary structures except for a small turn or 310 helix. The structure of nawaprin is very similar to elafin, a human leukocyte elastase-specific inhibitor. Although substantial parts of the nawaprin molecule are well defined, the tips of the outer and inner circular segments, which are hypothesized to be critical for binding interactions, are apparently disordered, similar to that found in elafin. The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom.

PubMed: 12878611
DOI: 10.1074/jbc.M305322200

実験手法

SOLUTION NMR