1UCP
NMR structure of the PYRIN domain of human ASC
1UCP の概要
エントリーDOI | 10.2210/pdb1ucp/pdb |
NMR情報 | BMRB: 5780 |
分子名称 | Apoptosis-associated speck-like protein containing a CARD (1 entity in total) |
機能のキーワード | death domain fold, apoptosis |
由来する生物種 | Homo sapiens (human) |
細胞内の位置 | Cytoplasm: Q9ULZ3 |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 10091.67 |
構造登録者 | Liepinsh, E.,Barbals, R.,Dahl, E.,Sharipo, A.,Staub, E.,Otting, G. (登録日: 2003-04-16, 公開日: 2003-11-04, 最終更新日: 2023-12-27) |
主引用文献 | Liepinsh, E.,Barbals, R.,Dahl, E.,Sharipo, A.,Staub, E.,Otting, G. The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition J.Mol.Biol., 332:1155-1163, 2003 Cited by PubMed Abstract: The PYRIN domain is a conserved sequence motif identified in more than 20 human proteins with putative functions in apoptotic and inflammatory signalling pathways. The three-dimensional structure of the PYRIN domain from human ASC was determined by NMR spectroscopy. The structure determination reveals close structural similarity to death domains, death effector domains, and caspase activation and recruitment domains, although the structural alignment with these other members of the death-domain superfamily differs from previously predicted amino acid sequence alignments. Two highly positively and negatively charged surfaces in the PYRIN domain of ASC result in a strong electrostatic dipole moment that is predicted to be present also in related PYRIN domains. These results suggest that electrostatic interactions play an important role for the binding between PYRIN domains. Consequently, the previously reported binding between the PYRIN domains of ASC and ASC2/POP1 or between the zebrafish PYRIN domains of zAsc and Caspy is proposed to involve interactions between helices 2 and 3 of one PYRIN domain with helices 1 and 4 of the other PYRIN domain, in analogy to previously reported homophilic interactions between caspase activation and recruitment domains. PubMed: 14499617DOI: 10.1016/j.jmb.2003.07.007 主引用文献が同じPDBエントリー |
実験手法 | SOLUTION NMR |
構造検証レポート
検証レポート(詳細版)
をダウンロード
