1UBI

SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1

1UBI の概要

• エントリーDOI: 10.2210/pdb1ubi/pdb
• 分子名称: UBIQUITIN (2 entities in total)
• 機能のキーワード: chromosomal protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8576.83

構造登録者

Alexeev, D.,Bury, S.M.,Turner, M.A.,Ogunjobi, O.M.,Muir, T.W.,Ramage, R.,Sawyer, L. (登録日: 1994-02-03, 公開日: 1994-05-31, 最終更新日: 2024-02-14)

主引用文献

Ramage, R.,Green, J.,Muir, T.W.,Ogunjobi, O.M.,Love, S.,Shaw, K.
Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin.
Biochem.J., 299:151-158, 1994

PubMed Abstract: The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used to authenticate the primary structure. Isoelectric focusing and m.s. were used to demonstrate that the final product was greater than 98% pure with a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol scale.

PubMed: 8166633

実験手法

X-RAY DIFFRACTION (1.8 Å)