1UB4

crystal structure of MazEF complex

Summary for 1UB4

• Entry DOI: 10.2210/pdb1ub4/pdb
• Descriptor: MazF protein, MazE protein (3 entities in total)
• Functional Keywords: toxin, antidote, programmed cell death, post-segregation, addiction module, structural genomics, psi, protein structure initiative, new york sgx research center for structural genomics, nysgxrc, dna binding protein
• Biological source: Escherichia coli; More
• Total number of polymer chains: 3
• Total formula weight: 33620.67

Authors

Kamada, K.,Hanaoka, F.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (deposition date: 2003-03-28, release date: 2003-05-20, Last modification date: 2023-12-27)

Primary citation

Kamada, K.,Hanaoka, F.,Burley, S.K.
Crystal Structure of the MazE/MazF Complex. Molecular Bases of Antidote-Toxin Recognition
Mol.Cell, 11:875-884, 2003

PubMed Abstract: A structure of the Escherichia coli chromosomal MazE/MazF addiction module has been determined at 1.7 A resolution. Addiction modules consist of stable toxin and unstable antidote proteins that govern bacterial cell death. MazE (antidote) and MazF (toxin) form a linear heterohexamer composed of alternating toxin and antidote homodimers (MazF(2)-MazE(2)-MazF(2)). The MazE homodimer contains a beta barrel from which two extended C termini project, making interactions with flanking MazF homodimers that resemble the plasmid-encoded toxins CcdB and Kid. The MazE/MazF heterohexamer structure documents that the mechanism of antidote-toxin recognition is common to both chromosomal and plasmid-borne addiction modules, and provides general molecular insights into toxin function, antidote degradation in the absence of toxin, and promoter DNA binding by antidote/toxin complexes.

PubMed: 12718874
DOI: 10.1016/S1097-2765(03)00097-2

Experimental method

X-RAY DIFFRACTION (1.7 Å)