1UAI

Crystal Structure of the Alginate Lyase from Corynebacterium sp.

1UAI の概要

• エントリーDOI: 10.2210/pdb1uai/pdb
• 分子名称: polyguluronate lyase (2 entities in total)
• 機能のキーワード: jellyroll beta-sandwich, lyase
• 由来する生物種: Corynebacterium sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24340.87

構造登録者

Kakuta, Y. (登録日: 2003-03-11, 公開日: 2004-07-27, 最終更新日: 2024-11-13)

主引用文献

Osawa, T.,Matsubara, Y.,Muramatsu, T.,Kimura, M.,Kakuta, Y.
Crystal structure of the alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. at 1.2 A resolution
J.Mol.Biol., 345:1111-1118, 2005

PubMed Abstract: The crystal structure of alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. (ALY-1) was determined at 1.2A resolution using the MAD method and bromide ions. The structure of ALY-1 is abundant in beta-strands and has a deep cleft, similar to the jellyroll beta-sandwich found in 1,3-1,4-beta-glucanase. The structure suggests that alginate molecules may penetrate into the cleft to interact with the catalytic site of ALY-1. The reported crystal structure of another type of alginate lyase, A1-III, differs from that of ALY-1 in that it consists almost entirely of alpha-helical structure. Nevertheless, the putative catalytic residues in both enzymes are positioned in space in nearly identical arrangements. This finding suggests that both alginate lyases may have evolved through convergent evolution.

PubMed: 15644208
DOI: 10.1016/j.jmb.2004.10.081

実験手法

X-RAY DIFFRACTION (1.2 Å)