1U9P

Permuted single-chain Arc

1U9P の概要

• エントリーDOI: 10.2210/pdb1u9p/pdb
• 分子名称: pArc (2 entities in total)
• 機能のキーワード: arc, parc, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13760.58

構造登録者

Tabtiang, R.K.,Cezairliyan, B.O.,Grant, R.A.,Cochrane, J.C.,Sauer, R.T. (登録日: 2004-08-10, 公開日: 2005-02-15, 最終更新日: 2024-02-14)

主引用文献

Tabtiang, R.K.,Cezairliyan, B.O.,Grant, R.A.,Cochrane, J.C.,Sauer, R.T.
Consolidating critical binding determinants by noncyclic rearrangement of protein secondary structure
Proc.Natl.Acad.Sci.Usa, 102:2305-2309, 2005

PubMed Abstract: We designed a single-chain variant of the Arc repressor homodimer in which the beta strands that contact operator DNA are connected by a hairpin turn and the alpha helices that form the tetrahelical scaffold of the dimer are attached by a short linker. The designed protein represents a noncyclic permutation of secondary structural elements in another single-chain Arc molecule (Arc-L1-Arc), in which the two subunits are fused by a single linker. The permuted protein binds operator DNA with nanomolar affinity, refolds on the sub-millisecond time scale, and is as stable as Arc-L1-Arc. The crystal structure of the permuted protein reveals an essentially wild-type fold, demonstrating that crucial folding information is not encoded in the wild-type order of secondary structure. Noncyclic rearrangement of secondary structure may allow grouping of critical active-site residues in other proteins and could be a useful tool for protein design and minimization.

PubMed: 15689399
DOI: 10.1073/pnas.0409562102

実験手法

X-RAY DIFFRACTION (1.9 Å)