1U73

Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu

1U73 の概要

• エントリーDOI: 10.2210/pdb1u73/pdb
• 関連するPDBエントリー: 1umv
• 分子名称: hypotensive phospholipase A2 (2 entities in total)
• 機能のキーワード: acidic phospholipase a2, bothrops jararacussu venom, platelet aggregation and hypotensive effects, oligomeric state, dimeric, hydrolase
• 由来する生物種: Bothrops jararacussu (jararacussu)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27400.90

構造登録者

Magro, A.J.,Murakami, M.T.,Soares, A.M.,Arni, R.K.,Fontes, M.R. (登録日: 2004-08-02, 公開日: 2004-10-12, 最終更新日: 2024-11-13)

主引用文献

Magro, A.J.,Murakami, M.T.,Marcussi, S.,Soares, A.M.,Arni, R.K.,Fontes, M.R.
Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A(2) in the monomeric and dimeric states: insights into its oligomeric state
Biochem.Biophys.Res.Commun., 323:24-31, 2004

PubMed Abstract: Phospholipases A2 belong to the superfamily of proteins which hydrolyzes the sn-2 acyl groups of membrane phospholipids to release arachidonic acid and lysophospholipids. An acidic phospholipase A2 isolated from Bothrops jararacussu snake venom presents a high catalytic, platelet aggregation inhibition and hypotensive activities. This protein was crystallized in two oligomeric states: monomeric and dimeric. The crystal structures were solved at 1.79 and 1.90 angstroms resolution, respectively, for the two states. It was identified a Na+ ion at the center of Ca2+-binding site of the monomeric form. A novel dimeric conformation with the active sites exposed to the solvent was observed. Conformational states of the molecule may be due to the physicochemical conditions used in the crystallization experiments. We suggest dimeric state is one found in vivo.

PubMed: 15351695
DOI: 10.1016/j.bbrc.2004.08.046

実験手法

X-RAY DIFFRACTION (1.9 Å)