1U5Z

The Crystal structure of murine APRIL, pH 8.5

1U5Z の概要

• エントリーDOI: 10.2210/pdb1u5z/pdb
• 関連するPDBエントリー: 1U5X 1U5Y
• 分子名称: Tumor necrosis factor ligand superfamily member 13, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: tnfsf, cytokine, trimer, jelly-roll, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Secreted (By similarity): Q9D777
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15763.73

構造登録者

Wallweber, H.J.,Compaan, D.M.,Starovasnik, M.A.,Hymowitz, S.G. (登録日: 2004-07-28, 公開日: 2004-10-12, 最終更新日: 2024-11-06)

主引用文献

Wallweber, H.J.,Compaan, D.M.,Starovasnik, M.A.,Hymowitz, S.G.
The Crystal Structure of A Proliferation-inducing Ligand, APRIL.
J.Mol.Biol., 343:283-290, 2004

PubMed Abstract: A proliferation-inducing ligand (APRIL) is a TNF-like cytokine that stimulates tumor cell growth. Within the TNF ligand superfamily, APRIL is most similar to B-cell activation factor (BAFF) with which it shares 30% sequence identity. APRIL binds the receptors B-cell maturation antigen (BCMA) and TACI with high affinity; both of these receptors have also been shown to bind BAFF, although BCMA has significantly higher affinity for APRIL than BAFF. Determination of the crystal structure of APRIL from three crystallization conditions at resolutions of 1.8-2.4A over a pH range from 5.0 to 8.5 reveals a compact trimeric ligand with a backbone fold very similar to that of BAFF (1.1A RMSD over 122 structurally equivalent Calpha atoms), with the exception of differences in the AA' and DE loop regions. Whereas BAFF has been shown to form 20-trimer assemblies under certain conditions, the molecular determinants required for BAFF-like assemblies are absent in the APRIL structure. No crystal packing suggestive of the formation of higher-order assemblies is seen in any of the crystal forms nor does the structure vary significantly between pH 5.0 and 8.5. Modeling of the APRIL-BCMA complex shows the resulting interface is in agreement with mutagenesis data.

PubMed: 15451660
DOI: 10.1016/j.jmb.2004.08.040

実験手法

X-RAY DIFFRACTION (2.4 Å)