1TXU

Crystal Structure of the Vps9 Domain of Rabex-5

1TXU の概要

• エントリーDOI: 10.2210/pdb1txu/pdb
• 分子名称: Rab5 GDP/GTP exchange factor, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: vps9 domain, rab5 guanine-nucleotide exchange factor, gef, protein transport
• 由来する生物種: Homo sapiens
• 細胞内の位置: Cytoplasm : Q9UJ41
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32388.09

構造登録者

Delprato, A.,Merithew, E.,Lambright, D.G. (登録日: 2004-07-06, 公開日: 2004-09-21, 最終更新日: 2024-11-06)

主引用文献

Delprato, A.,Merithew, E.,Lambright, D.G.
Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5
Cell(Cambridge,Mass.), 118:607-617, 2004

PubMed Abstract: The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.

PubMed: 15339665
DOI: 10.1016/j.cell.2004.08.009

実験手法

X-RAY DIFFRACTION (2.35 Å)