1TX7

Bovine Trypsin complexed with p-amidinophenylmethylphosphinic acid (AMPA)

1TX7 の概要

• エントリーDOI: 10.2210/pdb1tx7/pdb
• 分子名称: Trypsinogen, CALCIUM ION, (4-CARBAMIMIDOYLPHENYL)-METHYL-PHOSPHINIC ACID, ... (4 entities in total)
• 機能のキーワード: trypsin, bovine, p-amidinophenylmethylphosphinic acid (ampa), hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted, extracellular space: P00760
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23562.52

構造登録者

Cui, J.,Marankan, F.,Fu, W.,Crich, D.,Mesecar, A.,Johnson, M.E. (登録日: 2004-07-02, 公開日: 2005-09-20, 最終更新日: 2024-11-13)

主引用文献

Cui, J.,Marankan, F.,Fu, W.,Crich, D.,Mesecar, A.,Johnson, M.E.
An oxyanion-hole selective serine protease inhibitor in complex with trypsin.
Bioorg.Med.Chem., 10:41-46, 2002

PubMed Abstract: p-amidinophenylmethylphosphinic acid (AMPA) was designed, synthesized and crystallized in complex with trypsin to study interactions with the oxyanion hole at the S1 site. In comparison to benzamidine, AMPA shows improved activity, which the crystal structure demonstrates to result from hydrogen bonds between the negatively charged phosphinic acid group and the catalytic residues at the oxyanion hole.

PubMed: 11738605
DOI: 10.1016/S0968-0896(01)00259-0

実験手法

X-RAY DIFFRACTION (1.75 Å)