1TW4

Crystal Structure of Chicken Liver Basic Fatty Acid Binding Protein (Bile Acid Binding Protein) Complexed With Cholic Acid

1TW4 の概要

• エントリーDOI: 10.2210/pdb1tw4/pdb
• 関連するPDBエントリー: 1TVQ
• 分子名称: Fatty acid-binding protein, CHOLIC ACID (3 entities in total)
• 機能のキーワード: lb-fabp, fabp, babp, intracellular bile acid-binding protein, cholic acid, bile acid, lipid binding protein
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Cytoplasm: P80226
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29834.64

構造登録者

Nichesola, D.,Perduca, M.,Capaldi, S.,Carrizo, M.E.,Righetti, P.G.,Monaco, H.L. (登録日: 2004-06-30, 公開日: 2004-11-23, 最終更新日: 2024-03-13)

主引用文献

Nichesola, D.,Perduca, M.,Capaldi, S.,Carrizo, M.E.,Righetti, P.G.,Monaco, H.L.
Crystal structure of chicken liver basic Fatty Acid-binding protein complexed with cholic acid
Biochemistry, 43:14072-14079, 2004

PubMed Abstract: Two paralogous groups of liver fatty acid-binding proteins (FABPs) have been described: the mammalian type liver FABPs and the basic type (Lb-FABPs) characterized in several vertebrates but not in mammals. The two groups have similar sequences and share a highly conserved three-dimensional structure, but their specificity and stoichiometry of binding are different. The crystal structure of chicken Lb-FABP complexed with cholic acid and that of the apoprotein refined to 2.0 A resolution are presented in this paper. The two forms of the protein crystallize in different space groups, and significant changes are observed between the two conformations. The holoprotein binds two molecules of cholate in the interior cavity, and the contacts observed between the two ligands can help to explain the reason for this stoichiometry of binding. Most of the amino acids involved in ligand binding are conserved in other members of the Lb-FABP family. Since the amino acid sequence of the Lb-FABPs is more similar to that of the bile acid-binding proteins than to that of the L-FABPs, the possibility that the Lb-FABPs might be more appropriately called liver bile acid-binding proteins (L-BABPs) is suggested.

PubMed: 15518556
DOI: 10.1021/bi0489661

実験手法

X-RAY DIFFRACTION (2 Å)